Document 0581
 DOCN  M9550581
 TI    Contribution of ED-1- and CD-8-positive cells to the development of
       crescentic-type anti-GBM nephritis in rats.
 DT    9505
 AU    Hattori T; Nagamatsu T; Ito M; Suzuki Y; Department of Pharmacology,
       Faculty of Pharmacy, Meijo; University, Nagoya, Japan.
 SO    Nippon Jinzo Gakkai Shi. 1994 Nov;36(11):1228-39. Unique Identifier :
       AIDSLINE MED/95156857
 AB    The current studies were designed to identify which mononuclear
       leukocytes have an important role in the development of glomerular
       injury using rats with original-type (mild injury) and crescentic-type
       (severe injury) anti-glomerular basement membrane (GBM) nephritis. 1)
       Proteinuria was persistent in crescentic-type anti-GBM nephritis
       compared with original-type anti-GBM nephritis. Macrophages/monocytes
       (ED-1), cytotoxic/suppressor T cells (CD-8), interleukin-2-receptor
       (CD-25)-positive cells and Ia-positive cells accumulated remarkably and
       persisted for longer in crescentic-type nephritic glomeruli. 2) We then
       performed investigations using immunosuppressants. Cyclosporin A
       abrogated proteinuria more effectively than azathioprine in
       crescentic-type nephritis. However, plasma antibody titer and glomerular
       rat IgG deposition were equally reduced by both azathioprine and
       cyclosporin A. The increase in the numbers of ED-1-, CD-8- and
       CD-25-positive cells in nephritic glomeruli was completely inhibited by
       cyclosporin A, but inhibited only slightly by azathioprine. 3) There was
       a correlation between the degree of proteinuria and the number of ED-1-
       and CD-8-positive cells. It is likely that these cells are leukocytes
       that lead to glomerular injury in nephritis. 4) In additional
       experiments using monoclonal antibodies against macrophages/monocytes
       and cytotoxic/suppressor T cells, urinary protein excretion and
       accumulation of these cells were blunted in nephritic rats treated with
       these antibodies. These results suggest that ED-1- and CD-8-positive
       cells are involved in the development of crescentic-type anti-GBM
       nephritis.
 DE    Animal  Antibodies, Monoclonal/THERAPEUTIC USE  Basement
       Membrane/IMMUNOLOGY  CD8-Positive T-Lymphocytes/*IMMUNOLOGY
       Glomerulonephritis/*IMMUNOLOGY/THERAPY  IgG/METABOLISM
       Immunosuppressive Agents/PHARMACOLOGY/THERAPEUTIC USE  Kidney
       Glomerulus/IMMUNOLOGY  Leukocyte Count/DRUG EFFECTS
       Macrophages/*IMMUNOLOGY  Male  Monocytes/*IMMUNOLOGY  Rats  Rats,
       Sprague-Dawley  JOURNAL ARTICLE

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