Document 0585
 DOCN  M9550585
 TI    Genetically simpler bovine leukemia virus derivatives can replicate
       independently of Tax and Rex.
 DT    9505
 AU    Boris-Lawrie K; Temin HM; McArdle Laboratory for Cancer Research,
       University of Wisconsin; Medical School, Madison.
 SO    J Virol. 1995 Mar;69(3):1920-4. Unique Identifier : AIDSLINE
       MED/95156628
 AB    Retrovirus genomes have a conserved modular organization that consists
       of trans-acting gag, pol, and env genes that function through cis-acting
       sequences to replicate the RNA genome to the DNA provirus. Genetically
       more complex retroviruses also encode regulatory genes and cis-acting
       sequences that are essential for their replication. We sought to convert
       a more complex retrovirus into a simpler retrovirus derivative that can
       replicate. We constructed novel, hybrid retrovirus vectors to replicate
       the gag, pol, and env genes of the more complex bovine leukemia virus
       (BLV) in the absence of regulatory genes and cis-acting response
       sequences. Most of the cis-acting sequences involved in the replication
       and regulation of BLV were replaced by the cis-acting transcriptional
       control sequences of a simpler retrovirus, spleen necrosis virus. We
       found that the resulting hybrid BLV derivatives can replicate
       independently of BLV Tax and Rex and the Tax and Rex cis-acting response
       sequences, as measured by successive passages of virus on target cells,
       detection of provirus sequences, and analyses of provirus and
       encapsidated RNAs.
 DE    Genes, pX  *Genetic Vectors  Leukemia Virus, Bovine/*GROWTH &
       DEVELOPMENT/GENETICS  RNA, Viral/BIOSYNTHESIS  Support, U.S. Gov't,
       P.H.S.  Virus Replication  JOURNAL ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).