Document 0586
 DOCN  M9550586
 TI    Virions released from cells transfected with a molecular clone of human
       T-cell leukemia virus type I give rise to primary and secondary
       infections of T cells.
 DT    9505
 AU    Derse D; Mikovits J; Polianova M; Felber BK; Ruscetti F; Laboratory of
       Leukocyte Biology, National Cancer; Institute-Frederick Cancer Research
       and Development Center,; Maryland 21702-1201.
 SO    J Virol. 1995 Mar;69(3):1907-12. Unique Identifier : AIDSLINE
       MED/95156625
 AB    The ability of molecular clones of human T-cell leukemia virus type I
       (HTLV-I) to direct the synthesis of infectious virions has not
       previously been demonstrated. An HTLV-I provirus originating from an
       adult T-cell leukemia patient was cloned into a plasmid vector and is
       designated pCS-HTLV. This molecular clone was shown to direct the
       synthesis of viral mRNA and proteins in transiently transfected cells;
       in addition, virus structural proteins were released into the culture
       medium. Viral proteins were assembled into virions that sedimented at a
       buoyant density characteristic of retrovirus particles and whose
       morphology was verified by electron microscopy. Virions concentrated
       from transiently transfected cell supernatants were incubated with
       primary cord blood lymphocytes or with transformed T-cell lines to
       establish that these particles were infectious. Expression of spliced,
       viral mRNAs in the T-cell cultures after both primary and secondary
       infections with cell-free virus revealed that pCS-HTLV encodes an
       infectious provirus.
 DE    Base Sequence  Cloning, Molecular  DNA Primers/CHEMISTRY  Genes,
       Structural, Viral  Human  HTLV-I/*GROWTH & DEVELOPMENT/GENETICS  HTLV-I
       Infections/*MICROBIOLOGY  In Vitro  Molecular Sequence Data  RNA,
       Viral/ANALYSIS  Support, U.S. Gov't, P.H.S.  T-Lymphocytes/*MICROBIOLOGY
       Transfection  Virion  JOURNAL ARTICLE

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