Document 0587
 DOCN  M9550587
 TI    Infection by retroviral vectors outside of their host range in the
       presence of replication-defective adenovirus.
 DT    9505
 AU    Adams RM; Wang M; Steffen D; Ledley FD; Department of Cell Biology,
       Baylor College of Medicine, Houston,; Texas 77030.
 SO    J Virol. 1995 Mar;69(3):1887-94. Unique Identifier : AIDSLINE
       MED/95156622
 AB    Retrovirus infection is normally limited to cells within a specific host
       range which express a cognate receptor that is recognized by the product
       of the env gene. We describe retrovirus infection of cells outside of
       their normal host range when the infection is performed in the presence
       of a replication-defective adenovirus (dl312). In the presence of
       adenovirus, several different ecotropic vectors are shown to infect
       human cell lines (HeLa and PLC/PRF), and a xenotropic vector is shown to
       infect murine cells (NIH 3T3). Infectivity is demonstrated by
       5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) staining,
       selection with G418 for neomycin resistance, and PCR identification of
       the provirus in infected cells. Infectivity is quantitatively dependent
       upon both the concentration of adenovirus (10(6) to 10(8) PFU/ml) and
       the concentration of retrovirus. Infection requires the simultaneous
       presence of adenovirus in the retrovirus infection medium and is not
       stimulated by preincubation and removal of adenovirus from the cells
       before retrovirus infection. The presence of adenovirus is shown to
       enhance the uptake of fluorescently labeled retrovirus particles into
       cells outside of their normal host range, demonstrating that the
       adenovirus enhances viral entry into cells in the absence of the
       recognized cognate receptor. This observation suggests new opportunities
       for developing safe retroviral vectors for gene therapy and new
       mechanisms for the pathogenesis of retroviral disease.
 DE    Adenoviridae/*GROWTH & DEVELOPMENT  Animal  Defective Viruses/GENETICS
       DNA, Viral/GENETICS  Genes, env  *Genetic Vectors  Hela Cells  Human  In
       Vitro  Mice  Proviruses/GENETICS  Rats  Retroviridae/*GROWTH &
       DEVELOPMENT  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       Transduction, Genetic  *Virus Replication  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).