Document 0610
 DOCN  M9550610
 TI    Pimelautide or trimexautide as built-in adjuvants associated with an
       HIV-1-derived peptide: synthesis and in vivo induction of antibody and
       virus-specific cytotoxic T-lymphocyte-mediated response.
 DT    9505
 AU    Deprez B; Gras-Masse H; Martinon F; Gomard E; Levy JP; Tartar A; Faculte
       de Pharmacie, URA CNRS 1309, Institut Pasteur de Lille,; France.
 SO    J Med Chem. 1995 Feb 3;38(3):459-65. Unique Identifier : AIDSLINE
       MED/95156422
 AB    Covalent association of lipopeptidic immunostimulants is known to
       improve the immunogenicity of short peptides. In this paper, we describe
       the synthesis of four analytically pure immunogens, prepared by two
       different strategies, in which a hexadecameric peptide (V3) derived from
       the principal neutralizing domain of HIV-1 envelope glycoprotein was
       associated with two different murein-derived lauroyl-peptides,
       Pimelautide (RP 44102), or Trimexautide (RP 56142). The in vivo
       immunogenicity of these compounds was evaluated according to two
       different criteria: the ability to elicit a cellular-T cytotoxic (CTL
       response) and the ability to stimulate antibody response. Our studies
       show that one of our compounds (TrxSucV3) was able to efficiently induce
       a relevant virus-specific CTL response, while another one (PimSucV3) was
       able to stimulate a strong antibody response to the linked peptide, or
       to a co-injected protein. These results suggest that both activities
       rely on different structure-activity relationships and that such a
       chemically defined model of peptide vaccines may be used to selectively
       stimulate subpopulations of immunocompetent cells.
 DE    Adjuvants, Immunologic/*CHEMICAL SYNTHESIS  Amino Acid Sequence  Animal
       Cells, Cultured  HIV Antibodies/BIOSYNTHESIS/IMMUNOLOGY  HIV Envelope
       Protein gp120/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  Mice  Mice, Inbred BALB C
       Mice, Inbred C57BL  Mice, Inbred DBA  Molecular Sequence Data
       Oligopeptides/*CHEMICAL SYNTHESIS/IMMUNOLOGY  Peptide
       Fragments/*IMMUNOLOGY  Pimelic Acids/*CHEMICAL SYNTHESIS/IMMUNOLOGY
       Support, Non-U.S. Gov't  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY  JOURNAL
       ARTICLE

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