Document 0612
 DOCN  M9550612
 TI    Antiretroviral activity of furocoumarins plus UVA light detected by a
       replication-defective retrovirus.
 DT    9505
 AU    Miolo G; Tomanin R; De Rossi A; Dall'Acqua F; Zacchello F; Scarpa M;
       Department of Pharmaceutical Sciences, University of Padova,; Italy.
 SO    J Photochem Photobiol B. 1994 Dec;26(3):241-7. Unique Identifier :
       AIDSLINE MED/95156188
 AB    The replication defective retrovirus, pXM5(N2), was used for an easy,
       safe and reproducible test for the screening of furocoumarins with
       antiretroviral activity. High titer viral supernatants have been
       photomodified by UVA light (20 kJ m-2) in the presence of different
       concentrations of two psolarens (8-methoxypsoralen, 8-MOP and
       4,5',8-trimethylpsoralen, TMP) and one angelicin
       (4,6,4'-trimethylangelicin, TMA). At low concentrations (100-250 ng
       ml-1) 8-MOP and TMA did not show any significant antiviral activity,
       while TMP demonstrated a reduction of virus infectivity by one log at
       250 ng ml-1. At the highest concentration (5 micrograms ml-1), TMA and
       TMP reduced the virus titer by one and more than two logs, respectively,
       being, therefore, two and four times more active than 8-MOP. The most
       active compound, TMP, was further tested on HIV-1 viral supernatants.
       Total inactivation of the HIV-1 (200 SFU) was obtained in the presence
       of 1 microgram ml-1 of TMP and 20 kJ m-2 of UVA light. Our results
       support the validity of the N2 system to detect the antiretroviral
       activity of furocoumarins and suggest the potential of TMP in
       combination with UVA light against HIV-1.
 DE    Animal  Antiviral Agents/*PHARMACOLOGY  Cell Line  Comparative Study
       Coumarins/*PHARMACOLOGY  Defective Viruses/*DRUG
       EFFECTS/PHYSIOLOGY/RADIATION EFFECTS  Dose-Response Relationship, Drug
       Human  HIV-1/*DRUG EFFECTS/PHYSIOLOGY/RADIATION EFFECTS
       Methoxsalen/PHARMACOLOGY  Mice  Photosensitizing Agents/*PHARMACOLOGY
       Psoralens/PHARMACOLOGY  Retroviridae/*DRUG EFFECTS/PHYSIOLOGY/RADIATION
       EFFECTS  Support, Non-U.S. Gov't  Trioxsalen/PHARMACOLOGY  *Ultraviolet
       Rays  Virus Replication  3T3 Cells  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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