Document 0626
 DOCN  M9550626
 TI    Detection of human immunodeficiency virus type 1 (HIV-1) in heel prick
       blood on filter paper from children born to HIV-1-seropositive mothers.
 DT    9505
 AU    Nyambi PN; Fransen K; De Beenhouwer H; Chomba EN; Temmerman M;
       Ndinya-Achola JO; Piot P; van der Groen G; Institute of Tropical
       Medicine, Antwerp, Belgium.
 SO    J Clin Microbiol. 1994 Nov;32(11):2858-60. Unique Identifier : AIDSLINE
       MED/95155616
 AB    The human immunodeficiency virus type 1 (HIV-1) DNA PCR results of 94
       dried blood spot (DBS) samples on filter paper and corresponding venous
       blood in EDTA obtained from infants born to HIV-1-seropositive mothers
       were compared. In addition, the results of HIV-1 DNA PCR on DBS and the
       HIV-1 RNA PCR from plasma of 70 paired samples were compared. A 100%
       specificity and a 95% sensitivity for HIV-1 DNA PCR on DBS compared with
       results for venous blood were observed for the 94 paired samples. The
       results of the DBS HIV-1 DNA PCR and HIV-1 RNA PCR of 70 corresponding
       plasma samples correlated perfectly (100%). The DBS HIV-1 DNA PCR method
       proved reliable for HIV-1 detection.
 DE    Adult  Child, Preschool  Cohort Studies  *Disease Transmission, Vertical
       DNA, Viral/*BLOOD  Filtration  Human  HIV Seropositivity/*TRANSMISSION
       HIV-1/*ISOLATION & PURIF  Infant  Infant, Newborn  Polymerase Chain
       Reaction  RNA, Viral/BLOOD  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).