Document 0630
 DOCN  M9550630
 TI    Comparative quantitation of human cytomegalovirus DNA in blood
       leukocytes and plasma of transplant and AIDS patients.
 DT    9505
 AU    Gerna G; Furione M; Baldanti F; Sarasini A; Virus Laboratory, University
       of Pavia, Italy.
 SO    J Clin Microbiol. 1994 Nov;32(11):2709-17. Unique Identifier : AIDSLINE
       MED/95155588
 AB    A new method for the quantitation of human cytomegalovirus (HCMV) DNA
       was used to determine the levels of viral DNA in parallel in 120 blood
       leukocyte (leukoDNAemia) and plasma (plasmaDNAemia) samples from 8 heart
       or heart-lung transplant patients and 17 AIDS patients with disseminated
       HCMV infection. PlasmaDNAemia was consistently associated with
       leukoDNAemia in both groups of patients. However, at least in the
       transplant patients, plasmaDNAemia was not necessarily associated with
       clinical symptoms, appearing later and disappearing earlier than
       leukoDNAemia during the course of infection. Quantitative mean levels of
       leukoDNAemia were mostly higher than those of plasmaDNAemia in both
       transplant and AIDS patients. However, in the absence of antiviral
       treatment, plasmaDNAemia levels were significantly higher in AIDS
       patients than in transplant recipients, whereas leukoDNAemia levels were
       not significantly different between the two groups of patients. A
       significant correlation was found between leukoDNAemia and plasmaDNAemia
       in AIDS patients, as well as in transplant recipients, although to a
       lesser degree. However, from a diagnostic standpoint, quantitative
       determination of plasmaDNAemia appears to represent a much less
       sensitive parameter than that of leukoDNAemia (or antigenemia) for
       monitoring HCMV infections and antiviral treatment.
 DE    Acute Disease  Adult  AIDS-Related Opportunistic Infections/*VIROLOGY
       Comparative Study  Cytomegalovirus/GENETICS/*ISOLATION & PURIF
       Cytomegalovirus Infections/VIROLOGY  DNA, Viral/*BLOOD  Female  Heart
       Transplantation/ADVERSE EFFECTS  Heart-Lung Transplantation/ADVERSE
       EFFECTS  Human  Leukocytes/*VIROLOGY  Male  Middle Age  Passive
       Cutaneous Anaphylaxis  Support, Non-U.S. Gov't  Viremia/*VIROLOGY
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).