Document 0631
 DOCN  M9550631
 TI    Rapid screening for early detection of mother-to-child transmission of
       human immunodeficiency virus type 1.
 DT    9505
 AU    Cassol S; Butcher A; Kinard S; Spadoro J; Sy T; Lapointe N; Read S;
       Gomez P; Fauvel M; Major C; et al; British Columbia Centre for
       Excellence in HIV/AIDS, Saint Paul's; Hospital, Canada.
 SO    J Clin Microbiol. 1994 Nov;32(11):2641-5. Unique Identifier : AIDSLINE
       MED/95155575
 AB    The testing of dried blood spots (DBSs) for the presence of human
       immunodeficiency type 1 (HIV-1) proviral DNA by PCR was first described
       in 1991. The technology has proven to be particularly valuable for
       resolving the infection status in HIV-1-indeterminate infants born to
       HIV-1-seropositive mothers. To broaden the applicability of DBS PCR, we
       adapted it to a standardized, commercially available microwell plate
       amplification and detection kit, Amplicor HIV-1, produced by Roche
       Diagnostic Systems. The microwell assay is rapid and easy to perform and
       uses equipment that is readily available in routine diagnostic
       laboratories. The high level of performance of the assay was
       demonstrated in 1,168 duplicate tests performed on 584 DBSs from 178
       uninfected and 100 HIV-1-infected individuals, including 56 children
       with perinatally acquired HIV-1. Of 12 infants who were followed
       prospectively from birth, 3 (25%) were infected in utero (PCR positive
       at birth) and 9 (75%) were infected intrapartum (PCR negative, culture
       negative at birth). Overall, HIV-1 DNA was identified in 3 of 11 (27.3%)
       DBSs collected from infected infants during the first 4 days of life, 8
       of 9 (88.9%) DBSs collected between 10 and 15 days postpartum, and 166
       of 167 (99.4%) DBSs collected after 15 days of age. All 320 DBSs from
       uninfected children were PCR DNA negative. These findings indicate that
       use of the Roche microwell DBS PCR assay provides a powerful new
       approach for large-scale perinatal screening programs and
       population-based studies of vertical transmission.
 DE    Acquired Immunodeficiency Syndrome/*DIAGNOSIS/*TRANSMISSION  Adult
       Child  Child, Preschool  *Disease Transmission, Vertical  Double-Blind
       Method  DNA, Viral/*BLOOD  Female  Human  HIV-1/*GENETICS  Infant  Male
       Polymerase Chain Reaction  Reagent Kits, Diagnostic  Support, Non-U.S.
       Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).