Document 0635
 DOCN  M9550635
 TI    Differential transcriptional activation in vitro by NF-kappa B/Rel
       proteins.
 DT    9505
 AU    Lin R; Gewert D; Hiscott J; Terry Fox Molecular Oncology Group, Lady
       Davis Institute for; Medical Research, Jewish General Hospital,
       Montreal, Quebec,; Canada.
 SO    J Biol Chem. 1995 Feb 17;270(7):3123-31. Unique Identifier : AIDSLINE
       MED/95155399
 AB    Distinct NF-kappa B subunit combinations contribute to the specificity
       of NF-kappa B-mediated transcriptional activation and to the induction
       of multiple cytokine genes including interferon-beta (IFN-beta). To
       evaluate the regulatory influence of different homo- and heterodimers,
       NF-kappa B subunits were analyzed for transcriptional activity in vitro
       using test templates containing two types of NF-kappa B recognition
       elements (the human immunodeficiency virus type 1 enhancer and the
       IFN-beta-positive regulatory domain-II (PRDII) as well as IFN-beta
       PRDIII-PRDI-PRDII linked to the -56 minimal promoter of rabbit
       beta-globin. Recombinant NF-kappa B subunits (p50, p65, c-Rel, p52, and
       I kappa B alpha) and interferon regulatory factor 1 were produced from
       either Escherichia coli or baculovirus expression systems.
       Transcriptional analysis in vitro demonstrated that 1) various dimeric
       complexes of NF-kappa B differentially stimulated transcription through
       the human immunodeficiency virus enhancer or PRDII up to 20-fold; 2)
       recombinant I kappa B alpha specifically inhibited NF-kappa B-dependent
       transcription in vitro; and 3) different NF-kappa B complexes and
       interferon regulatory factor 1 cooperated to stimulate transcription in
       vitro through the PRDIII-PRDI-PRDII virus-inducible regulatory domains
       of the IFN-beta promoter. These results demonstrate the role of NF-kappa
       B protein dimerization in differential transcriptional activation in
       vitro and emphasize the role of cooperativity between transcription
       factor families as an additional regulatory level to maintain
       transcriptional specificity.
 DE    Animal  Baculoviridae  Base Sequence  Cloning, Molecular
       Cytokines/*BIOSYNTHESIS  DNA Primers  DNA-Binding Proteins/METABOLISM
       Escherichia coli  Gene Expression  *Gene Expression Regulation
       Globin/GENETICS  HIV Enhancer  HIV-1/GENETICS
       Interferon-beta/*BIOSYNTHESIS  Molecular Sequence Data  NF-kappa
       B/ANTAGONISTS & INHIB/*METABOLISM  Oligonucleotide Probes  Promoter
       Regions (Genetics)  Proto-Oncogene Proteins/*METABOLISM  Rabbits
       Recombinant Proteins/METABOLISM  Regulatory Sequences, Nucleic Acid
       Restriction Mapping  Support, Non-U.S. Gov't  Templates
       Trans-Activation (Genetics)  Transcription Factors/METABOLISM
       *Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).