Document 0643
 DOCN  M9550643
 TI    Improvement of the T-cell response to a non immunogenic peptide by its
       tandem association with a highly efficient T-helper peptide.
 DT    9505
 AU    Rouaix F; Gras-Masse H; Mazingue C; Ridel PR; Diesis E; Marguerite M;
       Estaquier J; Capron A; Tartar A; Auriault C; Centre d'Immunologie des
       Maladies Transmissibles et Allergiques,; Unite mixte INSERM U167-CNRS
       624, Institut Pasteur de Lille,; France.
 SO    Immunopharmacology. 1994 Nov-Dec;28(3):215-22. Unique Identifier :
       AIDSLINE MED/95155010
 AB    The 45-69 peptide, an helper T-cell epitope derived from the HIV nef
       protein is strongly immunogenic. A T-cell proliferative response was
       observed following immunization of Lou/M rats with 45-69 peptide
       administered in low dose and without any adjuvant. It is already known
       that the T-cell response to the 115-131 peptide of Sm28GST antigen, a
       protein of the parasite Schistosoma mansoni, requires the presence of a
       carrier of the use of peptidic constructs. We demonstrate here that a
       T-cell response against the 115-131 peptide can be obtained in the
       absence of adjuvant using peptidic constructs (115-45 and 45-115
       peptides) resulting from tandem synthesis of 115-131 and 45-69 peptides.
       A covalent association of both peptides is necessary, since the
       coinjection of 45-69 and 115-131 peptides is not sufficient to induce a
       detectable anti-115-131 T-cell response. The mutual orientation between
       the respective tandem peptides (45-115 and 115-45) is critical for the
       T-cell response. These peptidic constructs possess distinct properties
       of antigenicity and immunogenicity but both allowed to reveal the
       existence of a specific T-cell response normally undetectable using
       115-131 peptide alone. This immunopharmacological approach should be
       useful in the rational design and construction of vaccines.
 DE    Adjuvants, Immunologic/ADMINISTRATION & DOSAGE  Amino Acid Sequence
       Animal  Antigenic Determinants/GENETICS  Antigens, Helminth/GENETICS
       Gene Products, nef/GENETICS/IMMUNOLOGY  Lymphocyte Transformation  Male
       Molecular Sequence Data  Peptides/GENETICS/*IMMUNOLOGY  Rats  Rats,
       Inbred Lew  Schistosoma mansoni/GENETICS/IMMUNOLOGY
       T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

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