Document 0661
 DOCN  M9550661
 TI    [Detection of antibodies against the virus of enzootic bovine leukosis
       in serum and milk samples using an immunoblot]
 DT    9505
 AU    Bunger I; Khalaf H; Cripe C; Rimpler M; Staatlichen
       Veterinaruntersuchungsamt Hannover.
 SO    DTW Dtsch Tierarztl Wochenschr. 1994 Oct;101(10):402-5. Unique
       Identifier : AIDSLINE MED/95154202
 AB    A method for detection of antibodies against the enzootic bovine
       leukemia virus in single serum and milk samples, as well as in pooled
       milk samples, is described. After the electrophoretical separation of
       the antigen proteins with SDS-PAGE, a transfer onto an immobilizing
       membrane is done, followed by immunodetection based on the reaction of
       the leukemia virus antibodies with antigen proteins. The
       antibody-antigen complex is visualized by a second, biotinylated
       antibody and peroxidase-avidin. This system is developed for
       verification of ELISA-results in surveillance of official admitted herds
       free of leukosis. The threshold for positive samples is tested by the
       European reference serum E-4, Copenhagen, the dilution 1:25,000 is
       recognized as positive. The detected antigen-antibody reactions are
       determined by scanning and comparison with the MW-markers.
 DE    Animal  Antibodies, Viral/*ANALYSIS/BLOOD  Antigen-Antibody Reactions
       Cattle  English Abstract  Enzootic Bovine Leukosis/*DIAGNOSIS
       Enzyme-Linked Immunosorbent Assay  Immunoblotting  Leukemia Virus,
       Bovine/*IMMUNOLOGY  Milk/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).