Document 0672
 DOCN  M9550672
 TI    Comparison of activation marker and TCR V beta gene product expression
       by CD4+ and CD8+ T cells in peripheral blood and lymph nodes from
       HIV-infected patients.
 DT    9505
 AU    Ramzaoui S; Jouen-Beades F; Michot F; Borsa-Lebas F; Humbert G; Tron F;
       Service de Chirurgie Digestive, CHU Charles Nicolle, Rouen,; France.
 SO    Clin Exp Immunol. 1995 Feb;99(2):182-8. Unique Identifier : AIDSLINE
       MED/95153899
 AB    Since lymphoid organs constitute the site of active and progressive HIV
       disease, analysis of their lymphocytes may provide more accurate
       information on T cell abnormalities than that obtained from studying
       peripheral blood lymphocytes. The objective of this study was to compare
       the expressions of activation markers and T cell receptor (TCR) V beta
       gene products by CD4+ and CD8+ T cells in lymph nodes (LN) and
       peripheral blood (PB) from healthy individuals and asymptomatic
       HIV-infected patients to determine whether anomalies that could be
       identified at the HIV replication site could support the hypothesis of T
       cell activation by HIV-encoded antigens or superantigens. CD4+ and CD8+
       T cells in paired LN and PB obtained from six healthy controls and five
       asymptomatic HIV-infected individuals were analysed by flow cytometry,
       using anti-CD38, anti-HLA-DR and 13 anti-V beta MoAbs that cover,
       approximately, 45% of the T cell repertoire. Analysis of T cell
       activation marker expression indicated that the percentages of CD4+ and
       CD8+ T cells bearing CD38 or CD38 and HLA-DR molecules were higher in
       patients than in controls and, in patients, higher in LN than in PB.
       Comparison between the V beta repertoires of CD4+ and CD8+ T cells in LN
       and PB showed that, in each healthy individual, a limited number of V
       beta families expressed by CD4+ or CD8+ T cells had different
       repartition in LN and PB, whereas in each HIV+ patient, more V beta
       families exhibited different distributions and these differences
       recurred among certain V beta segments, such as V beta 5.3 and V beta 21
       in the CD4+ T cell population and V beta 5.2/5.3, V beta 12 and V beta
       21 in the CD8+ T cell population. Taken together, these data argue for a
       skewed TCR repertoire in HIV infection and sustained activation of T
       cells by HIV-encoded antigens at the site of HIV replication, and
       further demonstrate that a high proportion of CD4+ T cells are in an
       activation state that may, indirectly, participate in their functional
       abnormalities.
 DE    Adult  Antigens, CD/*BIOSYNTHESIS  Biological Markers/BLOOD  Comparative
       Study  CD4-Positive T-Lymphocytes/IMMUNOLOGY  CD8-Positive
       T-Lymphocytes/IMMUNOLOGY  Gene Expression/IMMUNOLOGY  Human  HIV
       Infections/*IMMUNOLOGY  Lymph Nodes/CYTOLOGY  Lymphocyte
       Transformation/*IMMUNOLOGY  Male  Receptors, Antigen, T-Cell,
       alpha-beta/*BIOSYNTHESIS/GENETICS  T-Lymphocyte Subsets/*IMMUNOLOGY
       JOURNAL ARTICLE

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