Document 0737
 DOCN  M9550737
 TI    Fine analysis of humoral antibody response to envelope glycoprotein of
       SIV in infected and vaccinated macaques.
 DT    9505
 AU    Silvera P; Flanagan B; Kent K; Rud E; Powell C; Corcoran T; Bruck C;
       Thiriart C; Haigwood NL; Stott EJ; National Institute for Biological
       Standards and Control, Potters; Bar, Hertfordshire, United Kingdom.
 SO    AIDS Res Hum Retroviruses. 1994 Oct;10(10):1295-304. Unique Identifier :
       AIDSLINE MED/95151367
 AB    To characterize the serological response to SIV envelope, induced by
       vaccination with different envelope immunogens or by SIV infection,
       plasma samples from 11 cynomolgus macaques infected with simian
       immunodeficiency virus (SIV) and from 16 macaques vaccinated with three
       different recombinant envelope proteins were analyzed by (1) ELISA,
       using a variety of antigens including overlapping peptides encompassing
       the entire sequence of the envelope protein of SIV, and (2) competition
       assays, using neutralizing monoclonal antibodies to SIV gp120. Seven
       regions of SIV envelope were predicted to be antigenic. Peptides
       representing four of these, in the second and third variable regions (V2
       and V3) and the fourth constant (C4) region of gp120 and the Gnann
       region of gp41, were recognized by the majority of sera from infected
       and vaccinated animals. Additional antigenic regions were identified in
       the first and fourth variable domains (V1 and V4) and the carboxy
       terminus (C5) of gp120 and in three additional regions of gp41. Most
       infected and vaccinated animals made antibodies that competed with the
       binding of the three conformational MAbs. Among the vaccinated groups,
       antibodies induced by vaccination with precursor glycoproteins (gp140 or
       gp160) recognized several additional gp120 epitopes when compared with
       antibodies induced by external glycoprotein gp130. Sera from infected
       animals showed a more restricted gp120 response (17 of 46 peptides
       recognized) compared to animals vaccinated with precursor glycoproteins
       (31 peptides recognized). The converse was true for antibodies to gp41.
       Sera from animals vaccinated with recombinant gp140, produced in insect
       cells, were the only group that failed to compete with the binding of
       conformational MAbs. Finally, the development of antibodies to specific
       epitopes of gp120 and gp41 revealed differences between long-term
       survivors and nonsurvivors, implying that responses to specific epitopes
       may be important in conferring resistance to disease progression.
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal  Antibodies,
       Viral/*BIOSYNTHESIS/BLOOD  Antibody Formation  Gene Products,
       env/CHEMISTRY/*IMMUNOLOGY  Macaca fascicularis  Molecular Sequence Data
       Neutralization Tests  Peptide Fragments/IMMUNOLOGY  Simian Acquired
       Immunodeficiency Syndrome/*IMMUNOLOGY  Support, Non-U.S. Gov't
       SIV/*IMMUNOLOGY  Vaccines, Synthetic/IMMUNOLOGY  Viral
       Vaccines/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).