Document 0738
 DOCN  M9550738
 TI    Isolation of simian immunodeficiency viruses from two sooty mangabeys in
       Cote d'Ivoire: virological and genetic characterization and relationship
       to other HIV type 2 and SIVsm/mac strains.
 DT    9505
 AU    Peeters M; Janssens W; Fransen K; Brandful J; Heyndrickx L; Koffi K;
       Delaporte E; Piot P; Gershy-Damet GM; van der Groen G; Department of
       Infection and Immunity, Institute of Tropical; Medicine, Antwerp,
       Belgium.
 SO    AIDS Res Hum Retroviruses. 1994 Oct;10(10):1289-94. Unique Identifier :
       AIDSLINE GENBANK/X78511
 AB    OBJECTIVE: To search for the presence of SIV in sooty mangabeys and
       other monkey species in Cote d'Ivoire, West Africa, and to compare viral
       isolates with HIV-2 strains from the same region. METHODS: Forty-three
       captive housed monkeys (28 African green monkeys, 6 sooty mangabeys, 6
       baboons, and 6 patas monkeys) were tested for the presence of HIV and
       SIV antibodies. Virus was isolated from the peripheral blood lymphocytes
       of seropositive animals and from HIV-2 antibody-positive patients
       originating from Cote d'Ivoire, Ghana, Senegal, and Belgium. Viruses
       were characterized by Western blot and radioimmunoprecipitation assay.
       Proviral DNA was amplified by PCR, cloned, and sequenced to construct a
       phylogenetic tree. RESULTS: One African green monkey and three sooty
       mangabeys had antibodies that cross-reacted with HIV-2. From two
       mangabeys lentiviruses were isolated and designated as SIVsmCI2 and
       SIVsmCI8. Serological, virological, and sequence data showed that these
       isolates are members of the HIV-2/SIVsm/SIVmac group of primate
       lentiviruses. Furthermore, in the phylogenetic tree, these two new
       viruses form a distinct subgroup that is equidistant to the HIV-2
       strains and the previously described SIVsm/SIVmac viruses. CONCLUSION:
       This study provides additional evidence that sooty mangabey monkeys can
       be infected with a lentivirus in their natural habitat. Within the SIVsm
       and SIVmac viruses extensive genetic variation is observed.
 DE    Acquired Immunodeficiency Syndrome/BLOOD/VIROLOGY  Animal  Antibodies,
       Viral/BLOOD  Base Sequence  Belgium  Cercocebus atys/*VIROLOGY
       Cercopithecus aethiops/VIROLOGY  Comparative Study  Cote d'Ivoire  DNA
       Primers  Erythrocebus patas/VIROLOGY  Ghana  Human  HIV Antibodies/BLOOD
       HIV-2/*GENETICS  Molecular Sequence Data  Papio/VIROLOGY  *Phylogeny
       Polymerase Chain Reaction  Senegal  Support, Non-U.S. Gov't
       SIV/CLASSIFICATION/*GENETICS/*ISOLATION & PURIF  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).