Document 0743
 DOCN  M9550743
 TI    Nef and Gag synthetic peptide priming of antibody responses to HIV type
       1 antigens in mice and primates.
 DT    9505
 AU    Vaslin B; Claverie JM; Benveniste O; Barre-Sinoussi FC; Dormont D;
       Centre de Recherches du Service de Sante des Armees,; Laboratoire de
       Neuropathologie Experimentale et Neurovirologie,; Fontenay-aux-Roses,
       France.
 SO    AIDS Res Hum Retroviruses. 1994 Oct;10(10):1241-50. Unique Identifier :
       AIDSLINE MED/95151361
 AB    T epitope mapping in human immunodeficiency virus proteins provides a
       useful tool for AIDS vaccine design. We have previously shown that four
       peptides selected from the Gag polyprotein of HIV-1 were able to prime
       mice for in vitro lymphoproliferative responses. These responses were
       shown to be MHC restricted, and a pool of these peptides was able to
       prime mice for a subsequent humoral response to HIV-1 Gag proteins. Here
       we show that two of these Gag peptides are able to prime the anti-HIV-1
       IgG response to heat-inactivated HIV-1 in B10Sc.Cr mice. Furthermore, we
       extended this study in the nonhuman primate model, and show efficient
       priming of the IgG response to heat-inactivated HIV-1 using the pool of
       four Gag peptides in baboons. Further mapping of nonself peptides is
       extended to the HIV-1 Nef protein. Three potential Nef T epitopes
       located at positions 137-145, 98-107, and 81-95 are also shown to prime
       the IgG response to HIV-1 in the mouse model, although T cell
       proliferation to recall peptides in vitro was not detectable. Although
       they have not yet been defined as major helper T epitopes in humans,
       using classic in vitro stimulation assays, the fact that most of them
       are able to prime IgG responses in animals without detectable in vitro
       proliferative responses does not rule out their functional helper
       capacity in humans.
 DE    Amino Acid Sequence  Animal  *Antibody Formation  Antigenic Determinants
       Comparative Study  Enzyme-Linked Immunosorbent Assay  Gene Products,
       gag/*IMMUNOLOGY  Gene Products, nef/*IMMUNOLOGY  HIV
       Antibodies/ANALYSIS/*BIOSYNTHESIS/BLOOD  HIV Antigens/*IMMUNOLOGY
       HIV-1/*IMMUNOLOGY  Mice  Mice, Inbred C57BL/IMMUNOLOGY  Molecular
       Sequence Data  Papio/IMMUNOLOGY  Peptide Fragments/CHEMICAL
       SYNTHESIS/*IMMUNOLOGY  Structure-Activity Relationship  Support,
       Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).