Document 0746
 DOCN  M9550746
 TI    Association of alterations in NF-kappa B moieties with HIV type 1
       proviral latency in certain monocytic cells.
 DT    9505
 AU    Oakes JW; Bagasra O; Duan L; Pomerantz RJ; Department of Medicine,
       Dorrance H. Hamilton Laboratories,; Jefferson Medical College, Thomas
       Jefferson University,; Philadelphia, Pennsylvania 19104.
 SO    AIDS Res Hum Retroviruses. 1994 Oct;10(10):1213-9. Unique Identifier :
       AIDSLINE MED/95151358
 AB    Human immunodeficiency virus type 1 (HIV-1) replication is controlled by
       a complex array of virally encoded and cellular proteins. A wide
       spectrum of levels of HIV-1 expression have been demonstrated in various
       cells, both in cell culture and in vivo. Molecular mechanisms leading to
       restricted HIV-1 replication may differ between certain cell types. It
       is now demonstrated that HIV-1 proviral latency in the monocytic cell
       line U1, in which only extremely low levels of HIV-1 expression are
       detected in the baseline unstimulated state, is associated with
       alterations in nuclear factor-kappa B (NF-kappa B) moieties demonstrated
       in these cells by electrophoretic mobility shift assays (EMSAs) and in
       situ UV cross-linking studies. A predominance of p50 NF-kappa B moieties
       and possibly p50 homodimers or closely related species, rather than the
       p50-p56 heterodimer of NF-kappa B that is the predominant NF-kappa B
       species in most T lymphocytic and monocytic cells, is demonstrated in
       the nuclei of U1 cells. This pattern of NF-kappa B-related moieties
       differs from the latently infected T lymphocytic cell line ACH-2, and
       from the U937 monocytic line, the parental cell line of the U1 cellular
       clone. As such, these data suggest that different proximal mechanisms
       may lead to restricted HIV-1 replication in various cell types.
 DE    Base Sequence  Binding Sites  Cell Line  Electrophoresis, Polyacrylamide
       Gel  Human  HIV-1/*PHYSIOLOGY  Molecular Sequence Data
       Monocytes/*PHYSIOLOGY/*VIROLOGY  NF-kappa B/*BIOSYNTHESIS/ISOLATION &
       PURIF  Oligonucleotide Probes  Proviruses/*PHYSIOLOGY  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  Virus Latency  JOURNAL ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).