Document 0751
 DOCN  M9550751
 TI    Absence of HIV-1 DNA in cartilage from HIV-positive patients.
 DT    9505
 AU    Bujia J; Zietz C; Randolph P; Wilmes E; Gurtler L; Department of
       Otorhinolaryngology, Klinikum Grosshadern, Munich,; Germany.
 SO    Eur Arch Otorhinolaryngol. 1994;251(6):347-9. Unique Identifier :
       AIDSLINE MED/95151324
 AB    Human immunodeficiency virus (HIV) infections are mainly transferred by
       blood, semen or organ transplantations. Since allogenic transplants have
       an established place in reconstructive surgery, the possibility of
       transferring HIV with such transplants has been a subject of much
       concern. Postmortem cartilage samples were obtained from eight
       HIV-infected patients and examined using the polymerase chain reaction
       in order to detect proviral HIV-1 DNA (gag, pol, env). Blood, brain and
       spleen samples were also obtained and used as positive controls. Results
       showed that no cartilage sample contained any HIV-DNA, whereas proviral
       sequences were clearly demonstrated in perichondrium from six patients.
       These findings indicate that HIV is not present in cartilage of
       HIV-infected patients, making HIV transmission through cartilage
       grafting improbable when transplants from HIV-negative donors are used.
 DE    Adult  Base Sequence  Brain/*VIROLOGY  Cadaver  Cartilage/VIROLOGY
       Connective Tissue/*VIROLOGY  DNA, Viral/ANALYSIS/BLOOD/*GENETICS  Female
       Gene Amplification  Human  HIV Seropositivity/BLOOD/DIAGNOSIS/*VIROLOGY
       HIV-1/*GENETICS/ISOLATION & PURIF  Male  Middle Age  Molecular Sequence
       Data  Polymerase Chain Reaction  Spleen/*VIROLOGY  JOURNAL ARTICLE

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