Document 0772
 DOCN  M9550772
 TI    Cytokine gene expression in HIV-infected intestinal mucosa.
 DT    9505
 AU    McGowan I; Radford-Smith G; Jewell DP; Department of Gastroenterology,
       Radcliffe Infirmary, Oxford, UK.
 SO    AIDS. 1994 Nov;8(11):1569-75. Unique Identifier : AIDSLINE MED/95151234
 AB    OBJECTIVE: Cytokine dysregulation has been implicated in AIDS
       pathogenesis and the gastrointestinal tract, containing approximately
       40% of the body's lymphoid tissue, is likely to act both as a reservoir
       of viral infection and a site for immune dysregulation. In this study
       evidence of cytokine dysregulation in intestinal mucosa has been sought
       using the reverse transcriptase polymerase chain reaction (RT-PCR) to
       amplify cytokine mRNA. METHODS: RT-PCR was performed on intestinal
       biopsies obtained from 50 HIV-infected patients and 31 controls. Tissue
       was obtained at diagnostic endoscopy and total RNA extracted using an
       RNAzol technique. Following RT, cDNA was amplified using primers
       specific for beta-actin, interleukin (IL)-1 beta, tumour necrosis factor
       (TNF)-alpha, interferon (IFN)-gamma, IL-2, IL-4, IL-10 and IL-13.
       RESULTS: There was a significant increase in the expression of the
       proinflammatory cytokines IL-1 beta and IFN-gamma in the HIV-infected
       compared with the control small intestinal samples (P < 0.01). IL-10 was
       significantly reduced in the respective groups' large intestine (P <
       0.02). The expression of IL-2 was also reduced in both the small and
       large intestinal HIV samples although this was not significant. IL-13
       mRNA was only detected in one control patient. CONCLUSIONS:
       Dysregulation of cytokine gene expression occurs in the intestinal
       mucosa of patients with HIV infection and is characterized by increased
       expression of proinflammatory cytokine mRNA. Further studies are needed
       to localize the cellular origin of such dysregulation and to quantify
       the degree of abnormality.
 DE    Base Sequence  Comparative Study  Cytokines/*BIOSYNTHESIS  DNA Primers
       *Gene Expression  Homosexuality, Male  Human  HIV
       Infections/*IMMUNOLOGY/METABOLISM  Interferon Type II/BIOSYNTHESIS
       Interleukins/BIOSYNTHESIS  Intestinal Mucosa/*IMMUNOLOGY/METABOLISM
       Intestine, Large  Intestine, Small  Male  Molecular Sequence Data
       Reference Values  RNA, Messenger/ANALYSIS/BIOSYNTHESIS  Support,
       Non-U.S. Gov't  Tumor Necrosis Factor/BIOSYNTHESIS  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).