Document 0776
 DOCN  M9550776
 TI    Productive infection of normal CD40-activated human B lymphocytes by
       HIV-1.
 DT    9505
 AU    Poulin L; Paquette N; Moir S; Lapointe R; Darveau A; Department of
       Microbiology, Laval University, Quebec, Canada.
 SO    AIDS. 1994 Nov;8(11):1539-44. Unique Identifier : AIDSLINE MED/95151230
 AB    OBJECTIVE: Antigen-driven B-cell proliferation and maturation occur in
       germinal centres present in lymphoid tissues. This process is highly
       dependent on functional interactions between B and T lymphocytes. In
       vitro activation of CD40 present on B cells mimics B cell-T interactions
       and allows the proliferation of normal Epstein-Barr virus (EBV)-negative
       B lymphocytes. In HIV-1-seropositive individuals, B cells become exposed
       to free viral particles and to infected T lymphocytes while migrating
       through germinal centres. The effect of HIV-1 viral exposure on
       CD40-activated B lymphocytes was therefore examined. METHODS: Freshly
       isolated B lymphocytes were cultured in vitro through activation of
       CD40. B-cell proliferation, HIV-1 infectivity and viral production were
       monitored following B-lymphocyte exposure to HIV-1. In addition,
       HIV-mediated fusion between infected B cells and uninfected CD4+ T
       lymphocytes was assessed in a coculture assay. RESULTS: EBV-negative,
       CD40-activated human B lymphocytes were directly infected by HIV-1. The
       infection significantly reduced their proliferation rate. Viral
       production was detected in B-cell culture supernatant. Numerous fusion
       events indicated that HIV-1 infection of B lymphocytes could spread to T
       lymphocytes following HIV-1-mediated fusion of these two cell types.
       CONCLUSION: In view of the importance of B cell-T cell interactions in
       the maintenance of a functional immune system, disruption of
       B-lymphocyte development could have direct implications on the course of
       AIDS progression.
 DE    Antigens, CD/*IMMUNOLOGY  Antigens, Differentiation,
       B-Lymphocyte/*IMMUNOLOGY  B-Lymphocytes/*IMMUNOLOGY/*VIROLOGY  Cells,
       Cultured  CD4-Positive T-Lymphocytes/IMMUNOLOGY  DNA/BIOSYNTHESIS  DNA,
       Viral/ANALYSIS/BIOSYNTHESIS  Fluorescent Antibody Technique  Genes, env
       Genes, gag  Genes, pol  Human  HIV Seronegativity/IMMUNOLOGY
       HIV-1/GENETICS/*IMMUNOLOGY/PHYSIOLOGY  Lymphocyte Transformation
       Polymerase Chain Reaction  Support, Non-U.S. Gov't
       T-Lymphocytes/IMMUNOLOGY  Virus Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).