Document 0785
 DOCN  M9550785
 TI    Polyclonal antiidiotypic antibodies mimicking gp120 of HIV-1.
 DT    9505
 AU    Sperlagh M; Hoxie J; Maruyama H; Stefano K; Gonzales-Scarano F; Prewett
       M; Liang S; Matsushita S; Herlyn D; Wistar Institute, Philadelphia,
       Pennsylvania.
 SO    Viral Immunol. 1994;7(2):61-9. Unique Identifier : AIDSLINE MED/95151136
 AB    Rabbit antiidiotypic antibodies (Ab2) were produced against anti-HIV-1
       antibody 0.5 beta (Ab1), which binds to gp120 of HIV-1 and shows
       virus-neutralizing activity. The Ab2 bound specifically to the Ab1 and
       their binding to Ab2 was inhibited by a recombinant fragment of gp120
       (PB1) or a peptide (residues 301-324 of gp120), both expressing the
       Ab1-defined epitope. The Ab2 induced in rats antiantiidiotypic
       antibodies (Ab3) that were Ab1-like in their binding reactivities to
       PB1, native gp120 or peptide and shared idiotopes with the Ab1. However,
       the Ab2 did not induce virus-neutralizing Ab3, probably a reflection of
       the low avidity of the Ab3 as compared to the Ab1.
 DE    Animal  Antibodies, Anti-Idiotypic/BIOSYNTHESIS/*IMMUNOLOGY  Antigenic
       Determinants/IMMUNOLOGY  Binding, Competitive/IMMUNOLOGY  Enzyme-Linked
       Immunosorbent Assay  Flow Cytometry  HIV Antibodies/*IMMUNOLOGY  HIV
       Envelope Protein gp120/*IMMUNOLOGY  HIV-1/*IMMUNOLOGY  IgG/*IMMUNOLOGY
       Neutralization Tests  Peptide Fragments/IMMUNOLOGY  Rabbits  Rats  Rats,
       Inbred Lew  Recombinant Proteins/IMMUNOLOGY  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).