Document 0786
 DOCN  M9550786
 TI    HIV-1 expression induced by anti-cancer agents in latently
       HIV-1-infected ACH2 cells.
 DT    9505
 AU    O'Brien MC; Ueno T; Jahan N; Zajac-Kaye M; Mitsuya H; Experimental
       Retrovirology Section, National Cancer Institute,; Bethesda, Maryland
       20892.
 SO    Biochem Biophys Res Commun. 1995 Feb 27;207(3):903-9. Unique Identifier
       : AIDSLINE MED/95169150
 AB    The expression of human immunodeficiency virus type 1 (HIV-1) in
       infected cells is induced (or enhanced) by a number of agents including
       phorbol myristate acetate (PMA), phytohemagglutinin (PHA), certain
       infectious agents, certain cytokines, and ultraviolet light. ACH2 cells
       represent latently HIV-1-infected T-cells, which produce only a low
       level of HIV-1 in vitro. We found that various anti-cancer agents
       including 5-azacytidine (5-AZC), 5-fluorouracil (5-FU), methotrexate,
       cytosine arabinoside, and vinblastine potentiated the expression of
       HIV-1 in ACH2 cells. There was no evidence of altered DNA methylation
       patterns in ACH2 cells cultured with 5-FU unlike with 5-AZC. The
       NF-kappa B binding activity was found to be enhanced in ACH2 cells
       exposed to 5-FU (but not in those exposed to 5-AZC) as assessed by the
       mobility shift assay using an oligonucleotide containing two NF-kappa B
       binding sites. These data suggest that the use of certain anti-cancer
       agents may induce (or enhance) the expression of HIV-1.
 DE    Antineoplastic Agents/*PHARMACOLOGY  Azacytidine/PHARMACOLOGY  Base
       Sequence  Cell Line  Cell Survival  Cytarabine/PHARMACOLOGY
       CD4-Positive T-Lymphocytes/*VIROLOGY  DNA/METABOLISM
       Fluorouracil/PHARMACOLOGY  HIV-1/*GROWTH & DEVELOPMENT
       Methotrexate/PHARMACOLOGY  Methylation  Molecular Sequence Data
       NF-kappa B/METABOLISM  Phytohemagglutinins/PHARMACOLOGY
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  Vinblastine/PHARMACOLOGY
       JOURNAL ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).