Document 0806
 DOCN  M9550806
 TI    Ovine lentivirus (maedi-visna virus) protein expression in sheep
       alveolar macrophages.
 DT    9505
 AU    Lujan L; Begara I; Collie D; Watt NJ; Department of Veterinary
       Pathology, Royal (Dick) School of; Veterinary Studies, University of
       Edinburgh, UK.
 SO    Vet Pathol. 1994 Nov;31(6):695-703. Unique Identifier : AIDSLINE
       MED/95167763
 AB    The expression of gag (p15, p25) and env gene products in ovine
       lentivirus-infected cells was studied in 20 adult Texel ewes
       seropositive to maedi-visna virus and 10 seronegative matched controls.
       Bronchoalveolar lavage was performed to recover alveolar cell pools from
       which cytocentrifuge preparations were made. Single and double
       immunocytochemical techniques were applied to study viral replication
       and coexpression of viral markers with markers for macrophages,
       lymphocytes, and major histocompatibility complex (MHC) class II.
       Aveolar macrophages of eight of 20 infected sheep (40%) were positive
       for viral protein expression. The percentage of positive macrophages
       varied from < 1% to 12% of the total population of macrophages. Viral
       protein expression was not detected in lymphocytes or other cell types.
       A relationship between virus-replicating macrophages and differential
       expression of MHC class II molecules, upregulated in ovine lentivirus
       infection, could not be established. Pathology was evaluated in nine
       infected ewes. Animals with the highest levels of positive cells had
       moderate or severe lymphoid interstitial pneumonia. However, sheep with
       similar degrees of lesions had lower percentages of positive macrophages
       or were negative for viral protein detection. These results support the
       idea that a partial or even a complete loss in the restriction mechanism
       of maedi-visna virus in lungs can occur in some individuals.
 DE    Animal  Female  Gene Products, env/*BIOSYNTHESIS  Gene Products,
       gag/*BIOSYNTHESIS  HLA-DQ Antigens/BIOSYNTHESIS  HLA-DR
       Antigens/BIOSYNTHESIS  Immunohistochemistry  Macrophages,
       Alveolar/PATHOLOGY/*VIROLOGY  Pneumonia, Progressive Interstitial, of
       Sheep/PATHOLOGY/*VIROLOGY  Sheep  Support, Non-U.S. Gov't  Virus
       Replication  Visna-Maedi Virus/*METABOLISM/PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).