Document 0815
 DOCN  M9550815
 TI    CD8 T-cell clones producing interleukin-5 and interferon-gamma in
       bronchial mucosa of patients with asthma induced by toluene
       diisocyanate.
 DT    9505
 AU    Maestrelli P; Del Prete GF; De Carli M; D'Elios MM; Saetta M; Di Stefano
       A; Mapp CE; Romagnani S; Fabbri LM; Institute of Occupational Medicine,
       University of Padova, Italy.
 SO    Scand J Work Environ Health. 1994 Oct;20(5):376-81. Unique Identifier :
       AIDSLINE MED/95167457
 AB    OBJECTIVES--The aims of the present study were to determine whether
       specific in vivo stimulation of asthmatics sensitized with toluene
       diisocyanate (TDI) induces the activation of T lymphocytes in bronchial
       mucosa and to characterize their phenotype and cytokine secretion
       profile. METHODS--Bronchial biopsies from two subjects with occupational
       asthma due to TDI were obtained 48 h after an asthmatic reaction induced
       by an inhalation challenge with TDI and after three months of no
       exposure to TDI, at the time when the subjects had recovered from their
       asthma. The fragments of bronchial mucosa were cultured in the presence
       of interleukin-2 so that the in vivo activated T cells present in the
       tissue would expand, and T blasts were then cloned under limiting
       dilution conditions. RESULTS--From the two 48-h specimens, 65 and 63
       T-cell clones were obtained. Most of the clones exhibited the CD8
       phenotype (82 and 83%). All of the CD8 clones produced interferon-gamma
       and 44% produced interleukin-5, but only 6% secreted interleukin-4 as
       well. Three months after the cessation of exposure, growing T cells
       could not be recovered from bronchial biopsies cultured in
       interleukin-2. CONCLUSIONS--The results suggest that, in sensitized
       subjects, exposure to TDI induces the activation of a subset of CD8
       lymphocytes producing interferon-gamma and interleukin-5.
 DE    Adult  Asthma/CHEMICALLY INDUCED/*IMMUNOLOGY  Biopsy  Bronchi/CYTOLOGY
       Bronchial Provocation Tests  Case-Control Studies  Cells, Cultured
       Clone Cells  CD8-Positive T-Lymphocytes/*SECRETION  Human
       Immunophenotyping  Interferon Type II/*SECRETION
       Interleukin-5/*SECRETION  Lymphocyte Transformation  Male  Middle Age
       Mucous Membrane/CYTOLOGY  Occupational Diseases/CHEMICALLY
       INDUCED/*IMMUNOLOGY  Support, Non-U.S. Gov't  Toluene
       2,4-Diisocyanate/*ADVERSE EFFECTS/DIAGNOSTIC USE  JOURNAL ARTICLE

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