Document 0827
 DOCN  M9550827
 TI    The effects of adrenocorticotropic hormone and heat stress on the
       distribution of lymphocyte populations in immature male chickens.
 DT    9505
 AU    Trout JM; Mashaly MM; Department of Poultry Science, Pennsylvania State
       University,; University Park 16802.
 SO    Poult Sci. 1994 Nov;73(11):1694-8. Unique Identifier : AIDSLINE
       MED/95166739
 AB    This study investigated the effects of heat stress and
       adrenocorticotropic hormone (ACTH) on the distribution of lymphocyte
       populations. Two experiments were conducted; each used 12-wk-old Cornell
       K-strain male chickens. In Experiment 1, birds were exposed to a
       temperature of 35 C, 6 h/d for 5 d and control birds were maintained at
       24 C. In Experiment 2, birds received daily i.m. injections of 50 IU/kg
       body weight of ACTH for 5 d and control birds received .9% saline. On
       Day 6, blood and spleens were collected and lymphocytes were isolated.
       Indirect immunofluorescent labeling of lymphocytes were performed to
       detect B lymphocytes, CD4+ cells, and CD8+ cells. Neither ACTH injection
       nor heat stress affected percentages of B lymphocytes in the blood or
       the spleen. The CD4+ cells decreased significantly in the blood of
       ACTH-injected and heat-stressed birds and significantly increased in the
       spleens of heat-stressed birds. The CD4+ cells in the spleens of
       ACTH-injected birds did not differ from controls. The CD8+ cells
       significantly declined in the blood following both ACTH injection and
       heat stress and significantly increased in the spleen of the
       ACTH-injected birds but did not differ from controls in the
       heat-stressed birds. These results indicate that stress factors lead to
       redistribution of different lymphocyte populations.
 DE    Animal  Chickens/*BLOOD  Corticotropin/*ADMINISTRATION & DOSAGE
       *CD4-Positive T-Lymphocytes  *CD8-Positive T-Lymphocytes  Heat/*ADVERSE
       EFFECTS  Injections, Intramuscular  Lymphocyte Count  Male
       Spleen/CYTOLOGY/DRUG EFFECTS  Stress  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).